Bilirubln interference with the KinetIc Jaff # { 233 } Method for Serum Creatinine
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چکیده
392 CLINICAL CHEMISTRY, Vol. 24, No. 2, 1978 In the case of copper and zinc, the authors are to be congratulated on the excellent reproducibility of the techniques used. The fact that different wash procedures gave somewhat different results need not be surprising if one assumes that the efficiency in nemoving contaminating metal varied with the procedure. A point of criticism is their use of an ionic detergent, sodium lauryl sulfate, because this is more likely to leach out bound metal from the hair than is a nonionic detergent. Furthermore, if the authors suspected that bound metal was being leached out by their washing procedure, surely the experiments should have been performed for various periods of time. The authors’ contention that “hair
منابع مشابه
Automated reaction-rate method for determination of serum creatinine with the CentrifiChem.
An automated kinetic method to determine creatinine in serum with the Jaff#{233} reaction is described. The method does not require deproteinization or adsorption of creatinine on Fuller’s earth or ion.exchange resin. A maximum of 29 serum samples, standards, and control sera can be assayed in less than 1.5 mm. Interferences by most of the known Jaff#{233}-positive serum constituents were studi...
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We evaluated and compared three analytical procedures for measuring creatinine by testing pooled sera and sera from individual human subjects. In two of the methods, an enzymic and a conventional colorimetric procedure, creatinine was separated from serum proteins with tungstic acid and detected at 520 nm after reaction with alkaline picrate (Jaff#{233} reagent). The amount of enzymaticaIly act...
متن کاملImproved method for specific determination of creatinine in serum and urine.
The Jaff#{233} reaction is commonly used to determine the concentration of creatinine in urine and serum by measuring the color produced when picric acid and sodium hydroxide solutions are combined with urine or protein-free filtrate from serum. As is well known, substances other than creatinine react to give positive error, the relative amount of interference usually being much greater in seru...
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A new kinetic method to determine creatimne in serum by using an alkaline 3,5-dinitrobenzoate solution was recently reported (1). Interference from pseudo-creatinine chromogens, bilirubin, or triacylglycerols was negligible. The results obtained with this kinetic spectrophotometric assay, performed with an Eppendorf ACP 5040 analyzer, agreed well with the procedure of Jaff#{233}, as modified by...
متن کاملCombinedEnzymic - Jaff # { 233 } Methodfor Determinationof Creatininein Serum
Concentrations of creatinine, as determined in serum by a method involving the combined use of creatinine amidohydrolase (EC 3.5.2.10) and alkaline sodium picrate were found to be factitiously low, owing to a reversal of the enzyme reaction. This effect could be eliminated by converting creatine, the product of the enzymic reaction, to creatine phosphate. The combined enzymic-Jaff#{233} method ...
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تاریخ انتشار 2005